Green Synthesis and Antibacterial Activity of Silver Nanoparticle Using Cressa Cretica Plant Extract
Cressa cretica is a small useful herb for treating asthma, bronchitis, dyspepsia, flatulence, colic, anorexia, anaemia, and diabetes and skin disease. Plant mediated synthesis of nanoparticles is a green chemistry approach that interconnects nanotechnology and plant biotechnology. In the present study, green synthesis of silver nanoparticles (AgNPs) was prepared using aqueous extract of Cressa cretica, reducing aqueous silver nitrate and evaluates its antibacterial activity. The synthesized AgNPs were characterized using Ultraviolet-Visible Spectrometer, Fourier Transform Infrared Spectroscopy, Particle size analyzer, Zeta potential analyzer. The antibacterial activity of colloidal AgNPs was evaluated against Gram (+) ve and Gram (-)ve bacteria such as Bacillus subtilis and Escherichia coli (E. coli) are using disc diffusion method. The synthesized AgNPs of UV-spectrum showed prominent peak at 426nm, the particle size and charge of the particle was determined as negatively charged spherical particles of around 106 nm. The observed zone of inhibition was 15mm and 11 mm against G (+)ve and G (-)ve bacteria respectively. The silver nanoparticle (AgNPs) of Cressa cretica plant extract showed significant antibacterial activity against Escherichia coli (E. coli) and Bacillus sp. in comparison to both AgNO3 and raw plant extracts. Moreover, the AgNPs prepared are safe to be discharged in the environment and possibly utilized as effective antibacterial agent as shown by our study.